KMID : 1138520080110010127
|
|
Journal of Pharmacopuncture 2008 Volume.11 No. 1 p.127 ~ p.141
|
|
Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods
|
|
Lee Jae-Eun
Kim Byoung-Woo Rhim Tae-Jin Kim Dong-Hee Kwon Ki-Rok
|
|
Abstract
|
|
|
Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat¡¯s epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water).
Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them.
Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially ), and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase (GPDH) on the concentrations of 0.1, . Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, , and especially on the concentration of alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of , but alcohol extracts of Chowiseungcheng-tang had it on all concentrations.
Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipoeytes proliferaton, lipolysis of adipocytes and localized fat accumulation than water extracts.
|
|
KEYWORD
|
|
Chowiseungcheng-tang, Alcohol extracts, Water extracts, Preadipocytes, Poliferation, Adipocytes, Lipolysis, Fat tissues
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|